In vitro selection of wheat callus tolerant to high levels of salt and plant regeneration
Identifieur interne : 003452 ( Main/Exploration ); précédent : 003451; suivant : 003453In vitro selection of wheat callus tolerant to high levels of salt and plant regeneration
Auteurs : N. Barakat [Arabie saoudite] ; H. Abdel-Latif [Arabie saoudite]Source :
- Euphytica [ 0014-2336 ] ; 1996-01-01.
Abstract
Summary: Embryogenic calli isolated from immature embryos of four wheat cultivars were subjected to three in vitro selection methods for salt tolerance. The effect of NaCl on the selected and unselected cell lines has been investigated. The results indicated that the relative growth rate of callus decreased as the concentration of NaCl increased in both callus lines. The selected callus line gave a higher growth weight in the presence of NaCl in the medium and was highly significant as compared with unselected callus line across medium protocols in all wheat cultivars. The dry weight of both kinds of callus lines of all wheat cultivars increased markedly with increasing NaCl concentration in most cases. The Na+ and Cl- contents of both callus lines were increased with increasing salinity levels while K+ content was decreased. The selected callus line of each cultivar at the same salinity level produced significant amounts of Na+, K+ and Cl- higher than the unselected callus line in most salinity levels. However, the unselected callus lines of the cultivars Giza-157 and Sakha-90 at the same salinity level produced significant amounts of K+ higher than the selected callus line in most salinity levels. The proline content of both kinds of callus lines for all wheat cultivars was increased with increasing salinity level. However, the selected callus line gave a significantly higher proline content than the unselected callus line in all wheat cultivars at the same Salinity level. Results from the in vitro selection for NaCl tolerance showed that the stepwise method of increasing NaCl in the medium was more effective for plant regeneration than other methods.
Url:
DOI: 10.1007/BF00021062
Affiliations:
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Barakat</name><affiliation wicri:level="1"><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Tissue Culture Laboratory, Agronomy & Range Science Department, College of Agriculture & Veterinary Medicine, King Saud University, Al-Gassim</wicri:regionArea><wicri:noRegion>Al-Gassim</wicri:noRegion></affiliation></author><author><name sortKey="Abdel Latif, H" sort="Abdel Latif, H" uniqKey="Abdel Latif H" first="H." last="Abdel-Latif">H. Abdel-Latif</name><affiliation wicri:level="1"><country xml:lang="fr">Arabie saoudite</country><wicri:regionArea>Tissue Culture Laboratory, Agronomy & Range Science Department, College of Agriculture & Veterinary Medicine, King Saud University, Al-Gassim</wicri:regionArea><wicri:noRegion>Al-Gassim</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Euphytica</title><title level="j" type="sub">International Journal of Plant Breeding</title><title level="j" type="abbrev">Euphytica</title><idno type="ISSN">0014-2336</idno><idno type="eISSN">1573-5060</idno><imprint><publisher>Kluwer Academic Publishers; http://www.wkap.nl</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1996-01-01">1996-01-01</date><biblScope unit="volume">91</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="127">127</biblScope><biblScope unit="page" to="140">140</biblScope></imprint><idno type="ISSN">0014-2336</idno></series><idno type="istex">F51508777556FBCA74B6687A44F7379F99E5AE10</idno><idno type="DOI">10.1007/BF00021062</idno><idno type="ArticleID">BF00021062</idno><idno type="ArticleID">Art1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-2336</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Embryogenic calli isolated from immature embryos of four wheat cultivars were subjected to three in vitro selection methods for salt tolerance. The effect of NaCl on the selected and unselected cell lines has been investigated. The results indicated that the relative growth rate of callus decreased as the concentration of NaCl increased in both callus lines. The selected callus line gave a higher growth weight in the presence of NaCl in the medium and was highly significant as compared with unselected callus line across medium protocols in all wheat cultivars. The dry weight of both kinds of callus lines of all wheat cultivars increased markedly with increasing NaCl concentration in most cases. The Na+ and Cl- contents of both callus lines were increased with increasing salinity levels while K+ content was decreased. The selected callus line of each cultivar at the same salinity level produced significant amounts of Na+, K+ and Cl- higher than the unselected callus line in most salinity levels. However, the unselected callus lines of the cultivars Giza-157 and Sakha-90 at the same salinity level produced significant amounts of K+ higher than the selected callus line in most salinity levels. The proline content of both kinds of callus lines for all wheat cultivars was increased with increasing salinity level. However, the selected callus line gave a significantly higher proline content than the unselected callus line in all wheat cultivars at the same Salinity level. Results from the in vitro selection for NaCl tolerance showed that the stepwise method of increasing NaCl in the medium was more effective for plant regeneration than other methods.</div></front></TEI><affiliations><list><country><li>Arabie saoudite</li></country></list><tree><country name="Arabie saoudite"><noRegion><name sortKey="Barakat, N" sort="Barakat, N" uniqKey="Barakat N" first="N." last="Barakat">N. Barakat</name></noRegion><name sortKey="Abdel Latif, H" sort="Abdel Latif, H" uniqKey="Abdel Latif H" first="H." last="Abdel-Latif">H. Abdel-Latif</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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